Enhanced DNA toehold exchange reaction on a chip surface to discriminate single-base changes.
نویسندگان
چکیده
In this study, a single-base change discrimination strategy was developed through enhanced DNA toehold exchange reaction on a chip surface. A DNA strand with single-base change (SNPs, insertion or deletion) at an arbitrary position was clearly discriminated with nucleic acid concentrations from 0.8 nM to 1 μM through this method.
منابع مشابه
Control of DNA strand displacement kinetics using toehold exchange.
DNA is increasingly being used as the engineering material of choice for the construction of nanoscale circuits, structures, and motors. Many of these enzyme-free constructions function by DNA strand displacement reactions. The kinetics of strand displacement can be modulated by toeholds, short single-stranded segments of DNA that colocalize reactant DNA molecules. Recently, the toehold exchang...
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ورودعنوان ژورنال:
- Chemical communications
دوره 50 91 شماره
صفحات -
تاریخ انتشار 2014